Dual Labeled Probes with locked nucleic acids

LocNA probes and primers contain Locked Nucleic Acid bases which improves the stability and performance of qPCR assays. These modified bases provide a 2'-O, 4'-C methylene bridge which effectively "locks" the 2' oxygen to the 4' carbon. LocNA probes and primers are the optimal choice when designing highly-specific, shorter probes with challenging target sequences. This novel approach has opened up a broad range of possibilities for researchers working in qPCR.

Benefits

• Greater stability in assays.
• Higher performance in assays.
• Allows for more flexibility and possibilities when working on challenging target sequences.
• Especially effective in target regions with a low GC percentage, AKA shorter target sequences.
• Enhanced accuracy, SNP, and hybridization.

Specifications

• Sequence length from 5 - 40 bases
• Up to 15 LocNA bases per probe are possible
• Only DNA bases are allowed at the 5' and 3' end
• HPLC purified by default
• Delivered lyophilized (dry down) or concentration adjusted in tubes
• Production takes 5 working days
• QC report incl. ESI
• Average synthesis yields of 15 nmole resp. 2 OD

Ordering Options

Clink the links below to visit the LocNA order page for either probes or primers.

Order

Order LocNA primers

Available dye-quencher combinations:

*Contact us for custom probe projects not shown below.

Additional Probe Options