NGS UDI Kits

NGS UDI Primer Sets for Multiplexing on Illumina

Unique Dual Index Primer Pairs

Eurofins Genomics' NGS UDI Primer Sets to eliminate misassignment by index hopping.

  • Ideal for all NGS libraries types including libraries produced with NEB adapters
  • Unique pairs of u5 and u7 high performance index primers
  • UDIs for standard indexing (8 nucleotides)
  • Very high performance also for low-plexity pools
  • Minimum edit distance of three bases or higher

Each primer pair has a concentration of 10µM provided liquid in 10µl for single use.

5` linker sequence for target-specific primers:
u5 5'-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3'
u7 5'-GACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3'

The general structure of the primers can be found in each plate assigment document below.

NGS UDI Primer Sets are delivered in a single-use fully-skirted 96-well PCR plate with a pierceable foil seal. Just select your required NGS UDI Primer Set with required quantity and press "Add to Cart"!

Kit Description Order Control

NGS UDI Primer Set 96-1


96 primer pairs for 96 reactions


Click here to download plate assignment with u7 and u5 index sequences and expected u7 and u5 index reads.

NGS UDI Primer Set 96-2


96 primer pairs for 96 reactions


Click here to download plate assignment with u7 and u5 index sequences and expected u7 and u5 index reads

NGS UDI Primer Set 48


48 primer pairs for 48 reactions


Click here to download plate assignment with u7 and u5 index sequences and expected u7 and u5 index reads.

NGS UDI Primer Set 24-1


24 primer pairs for 24 reactions


Click here to download plate assignment with u7 and u5 index sequences and expected u7 and u5 index reads.

NGS UDI Primer Set 24-2


24 primer pairs for 24 reactions


Click here to download plate assignment with u7 and u5 index sequences and expected u7 and u5 index reads.