| 16F | CGG TTA CCT TGT TAC GAC TT | 20 | 55.2 | 45 | $8.50 | | 1 |
| 16R | AGA GTT TGA TCC TGG CTC AG | 20 | 57.3 | 50 | $8.50 | | 2 |
| 3AOX | GCA AAT GGC ATT CTG ACA TCC | 21 | 57.9 | 48 | $8.50 | | 3 |
| 5AOX | GAC TGG TTC CAA TTG ACA AGC | 21 | 57.9 | 48 | $8.50 | | 4 |
| 96gIII | CCC TCA TAG TTA GCG TAA CG | 20 | 57.3 | 50 | $8.50 | | 5 |
| BGHrev | TAG AAG GCA CAG TCG AGG | 18 | 56.0 | 56 | $8.50 | | 6 |
| CMVf | CGC AAA TGG GCG GTA GGC GTG | 21 | 65.7 | 67 | $8.50 | | 7 |
| CYC1Reverse | GCG TGA ATG TAA GCG TGA C | 19 | 56.7 | 53 | $8.50 | | 8 |
| EF-1alphaFor | TCA AGC CTC AGA CAG TGG TTC | 21 | 59.8 | 52 | $8.50 | | 9 |
| EGFPC1F | GAT CAC TCT CGG CAT GGA C | 19 | 58.8 | 58 | $8.50 | | 10 |
| EGFPC1R | CAT TTT ATG TTT CAG GTT CAG GG | 23 | 57.1 | 39 | $8.50 | | 11 |
| EGFPN1F | GTC GTA ACA ACT CCG CCC | 18 | 58.2 | 61 | $8.50 | | 12 |
| EGFPN1R | GTC CAG CTC GAC CAG GAT G | 19 | 61.0 | 63 | $8.50 | | 13 |
| GadFor | GGG ATG TTT AAT ACC ACT AC | 20 | 53.2 | 40 | $8.50 | | 14 |
| GadRev | AAG AAA TTG AGA TGG TGC AC | 20 | 53.2 | 40 | $8.50 | | 15 |
| Gal4AD | TAC CAC TAC AAT GGA TG | 17 | 47.9 | 41 | $8.50 | | 16 |
| Gal4BD | TCA TCG GAA GAG AGT AG | 17 | 50.4 | 47 | $8.50 | | 17 |
| GAPSEQF | GTT CGA CCC CGC CTC GAT CC | 20 | 65.5 | 70 | $8.50 | | 18 |
| GAPSEQR | TGA CAC ACA TTC CAC AGG GTC | 21 | 59.8 | 52 | $8.50 | | 19 |
| GL2 | CTT TAT GTT TTT GGC GTC TTC CA | 23 | 57.1 | 39 | $8.50 | | 20 |
| hU6F | GAG GGC CTA TTT CCC ATG ATT | 24 | 54.6 | 47.6 | $8.50 | | 78 |
| ITS1 | TCC GTA GGT GAA CCT GCG G | 19 | 61.0 | 63 | $8.50 | | 21 |
| ITS4 | TCC TCC GCT TAT TGA TAT GC | 20 | 55.2 | 45 | $8.50 | | 22 |
| M13(-96) | TGA GTT TCG TCA CCA GTA | 18 | 51.4 | 44 | $8.50 | | 23 |
| M13F | TGT AAA ACG ACG GCC AGT | 18 | 53.7 | 50 | $8.50 | | 24 |
| M13F43 | AGG GTT TTC CCA GTC ACG ACG TT | 23 | 62.4 | 52 | $8.50 | | 25 |
| M13R | CAG GAA ACA GCT ATG ACC | 18 | 53.7 | 50 | $8.50 | | 26 |
| M13R49 | GAG CGG ATA ACA ATT TCA CAC AGG | 24 | 61.0 | 46 | $8.50 | | 27 |
| malE | GGT CGT CAG ACT GTC GAT GAA GCC | 24 | 66.1 | 58 | $8.50 | | 28 |
| mCherry-F | CCC CGT AAT GCA GAA GAA GA | 20 | 57.3 | 50 | $8.50 | | 29 |
| mCherry-R | TTG GTC ACC TTC AGC TTG G | 19 | 56.7 | 53 | $8.50 | | 30 |
| Myc | GCA TCA ATG CAG AAG CTG ATC TCA | 24 | 61.0 | 46 | $8.50 | | 31 |
| pBABE3 | ACC CTA ACT GAC ACA CAT TCC | 21 | 57.9 | 48 | $8.50 | | 32 |
| pBABE5 | CTT TAT CCA GCC CTC AC | 17 | 52.8 | 53 | $8.50 | | 33 |
| pBabeF | TGA CCT GGG AAG CCT TGG CT | 20 | 61.4 | 60 | $8.50 | | 34 |
| pBabeR | TTG CTG ACT AAT TGA GAT GCA TGC TTT | 27 | 60.4 | 37 | $8.50 | | 35 |
| pJETSEQF | CGA CTC ACT ATA GGG AGA GCG GC | 23 | 66.0 | 61 | $8.50 | | 36 |
| pJETSEQR | AAG AAC ATC GAT TTT CCA TGG CAG | 24 | 59.3 | 42 | $8.50 | | 37 |
| pEXF | GGA GCA GAC AAG CCC GTC AGG | 21 | 65.7 | 67 | $8.50 | | 38 |
| pEXR | CAG GCT TTA CAC TTT ATG CTT CCG GC | 26 | 64.8 | 50 | $8.50 | | 39 |
| pBadF | ATG CCA TAG CAT TTT TAT CC | 20 | 51.1 | 35 | $8.50 | | 40 |
| pBadR | GAT TTA ATC TGT ATC AGG | 18 | 46.9 | 33 | $8.50 | | 41 |
| pcDNAF | GGC TAA CTA GAG AAC CCA CTG | 21 | 59.8 | 52 | $8.50 | | 42 |
| pcDNAR | GGC AAC TAG AAG GCA CAG TC | 20 | 59.3 | 55 | $8.50 | | 43 |
| petup | ATG CGT CCG GCG TAG A | 16 | 54.3 | 63 | $8.50 | | 44 |
| pFBACf | TCC GGA TTA TTC ATA CCG TCC C | 22 | 60.3 | 50 | $8.50 | | 45 |
| pFBACr | CCT CTA CAA ATG TGG TAT GGC TG | 23 | 60.6 | 48 | $8.50 | | 46 |
| pGexF | ATA GCA TGG CCT TTG CAG G | 19 | 56.7 | 53 | $8.50 | | 47 |
| pGexR | GAG CTG CAT GTG TCA GAG G | 19 | 58.8 | 58 | $8.50 | | 48 |
| pGL3for | CTA GCA AAA TAG GCT GTC CC | 20 | 57.3 | 50 | $8.50 | | 49 |
| pGLfor | GTA TCT TAT GGT ACT GTA ACT G | 22 | 54.7 | 36 | $8.50 | | 50 |
| pGLrev | CTT TAT GTT TTT GGC GTC TTC C | 22 | 56.5 | 41 | $8.50 | | 51 |
| pJET12F | CGA CTC ACT ATA GGG AGA GCG GC | 23 | 66.0 | 61 | $8.50 | | 52 |
| pJET12R | AAG AAC ATC GAT TTT CCA TGG CAG | 24 | 59.3 | 42 | $8.50 | | 53 |
| pJET1F | GCC TGA ACA CCA TAT CCA TCC | 21 | 59.8 | 52 | $8.50 | | 54 |
| pJET1R | GCA GCT GAG AAT ATT GTA GGA GAT C | 25 | 61.3 | 44 | $8.50 | | 55 |
| pQEfor | GTA TCA CGA GGC CCT TTC GTC T | 22 | 62.1 | 55 | $8.50 | | 56 |
| pQErev | CAT TAC TGG ATC TAT CAA CAG GAG | 24 | 59.3 | 42 | $8.50 | | 57 |
| pShCMVf | GGT CTA TAT AAG CAG AGC TG | 20 | 55.2 | 45 | $8.50 | | 58 |
| pShCMVr | GTG GTA TGG CTG ATT ATG ATC AG | 23 | 58.9 | 44 | $8.50 | | 59 |
| RV3 | CTA GCA AAA TAG GCT GTC CCC | 21 | 59.8 | 52 | $8.50 | | 60 |
| SeqL-A | TCG CGT TAA CGC TAG CAT GGA TCT C | 25 | 64.6 | 52 | $8.50 | | 61 |
| SeqL-B | GTA ACA TCA GAG ATT TTG AGA CAC | 24 | 57.6 | 38 | $8.50 | | 62 |
| SP6 | CAT TTA GGT GAC ACT ATA G | 19 | 50.2 | 37 | $8.50 | | 63 |
| SV40pA-R | GAA ATT TGT GAT GCT ATT GC | 20 | 51.1 | 35 | $8.50 | | 64 |
| SV40-promoter | TAT TTA TGC AGA GGC CGA GG | 20 | 57.3 | 50 | $8.50 | | 65 |
| T3 | AAT TAA CCC TCA CTA AAG GG | 20 | 53.2 | 40 | $8.50 | | 66 |
| T7 | TAA TAC GAC TCA CTA TAG GG | 20 | 53.2 | 40 | $8.50 | | 67 |
| T7EEV | ATG TCG TAA TAA CCC CGC CCC G | 22 | 64.0 | 59 | $8.50 | | 68 |
| T7PCS2 | TGT CTG GAT CTA CGT AAT ACG | 21 | 55.9 | 43 | $8.50 | | 69 |
| T7term | CTA GTT ATT GCT CAG CGG T | 19 | 54.5 | 47 | $8.50 | | 70 |
| TrcHisF | AAT CTG TGT GGG CAC TCG | 18 | 56.0 | 56 | $8.50 | | 71 |
| TrcHisR | CTT CTG CGT TCT GAT TTA ATC TG | 23 | 57.1 | 39 | $8.50 | | 72 |
| V1-5 | GGA CTT TCC AAA ATG TCG | 18 | 51.4 | 44 | $8.50 | | 73 |
| V5 | ACC GAG GAG AGG GTT AGG GA | 20 | 61.4 | 60 | $8.50 | | 74 |
| VF2 | TGC CAC CTG ACG TCT AAG AA | 20 | 57.3 | 50 | $8.50 | | 75 |
| VR | ATT ACC GCC TTT GAG TGA GC | 20 | 57.3 | 50 | $8.50 | | 76 |
| XL39 | ATT AGG ACA AGG CTG GTG GG | 20 | 59.3 | 55 | $8.50 | | 77 |
