Eurofins Genomics offers free subcloning for genes we synthesize into a standard cloning vector. Our standard vectors have been modified to minimize redundant restriction sites in order to facilitate downstream applications. Vector selection is performed by our team of specialists, and is dictated by the size of the insert and the complexity of the synthesis approach adopted.
||Primers for seq.
||No Cut Sites
||T7 / M13
||pEX-for / pEX-Rev
For a full listing of the standard cloning vectors we offer please contact our technical support team at GenomicsSupport@eurofins.com.
Eurofins Genomics also offers subcloning services into any cloning or expression vector of your choosing. Simply send us the vector into which you want us to subclone your insert or give us the name of the vendor and catalogue number and we will purchase the vector on your behalf for subcloning. Please contact our technical support team for more information on our custom subcloning services.
When sending a vector for subcloning, please provide at least 3 µg of vector DNA, lyophilized or in solution (conc.: ~200ng/µl). Use a padded envelope and state the order number clearly, and mail your vector and a copy of your quotation or order confirmation to:
Attn: Gene Synthesis Laboratory
12701 Plantside Drive,
Louisville, KY 40299
Please include the following information with the submission:
- Map of the plasmid
- Detailed description of the plasmid (incl. info about sequencing primers)
- Complete DNA sequence in text format
- If it is an unmodified commercially available plasmid: supplier and info if it is the original plasmid
- If it is a modified plasmid: detailed description of the modifications (incl. ab1 files and gel picture)