Tube Sequencing

New! 8-strip PCR tube ordering (details below)

97.3% success rate across all applications

Eurofins Genomics provides a fast and convenient way to sequence plasmids and PCR products. Our automated DNA sequencing services are your best choice for fast and reliable DNA sequencing. Our automated services allow rapid processing of for all template types either in tubes or 96-well plates. Let Eurofins Genomics handle your daily DNA sequencing work so you can return to what matters most: research in the lab. 

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“Ordering sequencing reactions through Eurofins is fast and easy.”

-          J. Chalupny, PhD

Tube Types

 

Barcoded Tubes

  • 1.5 – 2.0 mL Centrifuge tubes, with or without barcodes.
  • Fastest turnaround time, along with SimpleSeq tubes. Required for overnight sequencing.
  • Eurofins Genomics provides bar-code labels for use with templates and primers that are submitted for DNA sequencing services. By using bar-coded labels, you guarantee that each sample and primer submitted to Eurofins Genomics for DNA sequencing has a unique identifier that is tracked throughout our automated system.
  • Submitting online: Enter the barcode on the tube submission page. All tube types use the same sequencing tube submission page.
   

Non-Barcoded Tubes

  • 1.5 – 2.0 mL Centrifuge tubes, with or without barcodes.
  • Non-barcoded tubes are processed after barcoded tubes, so the turnaround time might be impacted slightly.
  • Additional fees may apply for not using barcodes as it requires more labor in the lab to track samples. This depends on your institutional agreement with Eurofins Genomics.
  • Submitting online: All tube types use the same sequencing tube submission page.
   

8 Strip PCR Tubes

  • Variety of sizes. 
  • If submitting less than 8, please cut the strip and save the remaining tubes
  • Submitting online (new process): 8-strip barcodes are automatically generated by our system and displayed on the online order page. Please write these barcodes on the side of your tubes using a sharpie marker. If using the excel upload form, please leave the barcode field empty. The barcodes are a combination of your initals and a number sequence, such as DR01...DR02...et cetera. All tube types use the same sequencing tube submission page.
   

SimpleSeq Tubes 

  • Pre-labeled
  • Tube size is 0.75 mL
  • All tubes provided with kit
  • Fastest turnaround time, along with normal barcoded tubes.
  • Submitting online: All you need to do is type in the barcode when submitting online. All tube types use the same sequencing tube submission page, except for SimpleSeq Premixed which requires no online entry.

 

Benefit of Barcodes

Barcodes are used to track samples and primers in the lab. Customers who use barcodes pay a lower price and receive a faster turnaround time. Customers enter these barcodes on the online submission page(s) to link the order to the tubes. By using bar-coded labels, you guarantee that each sample and primer submitted to Eurofins Genomics for DNA sequencing has a unique identifier that is tracked throughout our automated system.

Eurofins Genomics provides barcode labels for free. These barcodes can be applied to sample tubes and/or primer tubes. Free barcodes can be ordered on the supplies page.  In addition, all SimpleSeq kits come with prelabeled tubes and extra barcodes for primer tubes.

Submitting Barcodes on the Online Order Page

  • Standard and SimpleSeq barcodes can only contain digits. Please remove any hyphens, dashes, spaces, or periods before using a barcode. 
  • For assessing mutations in the sequence data using a trace decomposition assay (e.g., TIDE assay), please enter "TIDE Protocol" in the order comments. 
  • You do not need to order physical barcode labels if choosing 8-strip PCR tubes.  

Need help finding your barcode?  CLICK HERE to open your Sequencing Dashboard which easily displays all barcodes in your account.

 

Additional Information

  • Always adhere to sample submission guidelines to ensure that you receive the best sequence data with the shortest turnaround time.
  • Read lengths are typically >1000 although obviously results depend on sample and primer quality
  • Specific primers can be requested or enclosed
  • Over 80 free standard primers available
  • Template and primer may be sent premixed
  • Accuracy of data is greater than 99% (Q20 or better)
  • Prepayment options are available not only through SimpleSeq, but also with EVOcards

Submit Tubes