Overview

Over the years, our scientists have sequenced millions of reactions and gathered a list of standard primers which work well in a variety of templates. These universeral primers can be ordered either 1.) on the sequencing submission page for free or 2.) beforehand, using the one-click order page below, for a flat fee.  The purpose of ordering a standard primer beforehand is two-fold.  One, it allows researchers to premix their samples to their desired specifications. Premixed samples are processed first which ensures the earliest possible release of results. Two, one standard primer can be used for >300 reactions, which is very economical. 

Specifications

Standard primers are synthesized at the 50 nmole scale, HPSF purified, and can be used in hundreds of sequencing reactions. Our internal studies have shown that the HPSF-purified sequencing primers generate high quality chromatograms. Typically, the quality of the primer is not critical for the amplification of sequences of interest when the amplified PCR product is analyzed by agarose gel electrophoresis. On the contrary, when the primers are used for sequencing a template, the quality of the primers is critical for the sucess.

Mutations are commonly identified by direct sequencing of the PCR product. The primer quality is therefore even more critical when you want to detect or verify single base variants:

• Genotyping by sequencing
• Microsatellite analysis
• Verification of Site-Directed Mutagenesis experiments

The standard sequencing primers shown below have the following predefined parameters:

*please add the shipping days to the production days to determined the delivery days.

Ordering

To order, simply select the checkbox for the predefined sequence below and hit add to cart.