Standard Primers Information (click to expand)

Overview

Over the years, our scientists have sequenced millions of reactions and gathered a list of standard primers which work well in a variety of templates. These universeral primers can be ordered either 1.) on the sequencing submission page for free or 2.) beforehand, using the one-click order page below, for a flat fee.  The purpose of ordering a standard primer beforehand is two-fold.  One, it allows researchers to premix their samples to their desired specifications. Premixed samples are processed first which ensures the earliest possible release of results. Two, one standard primer can be used for >300 reactions, which is very economical. 

Specifications

Standard primers are synthesized at the 50 nmole scale, HPSF purified, and can be used in hundreds of sequencing reactions. Our internal studies have shown that the HPSF-purified sequencing primers generate high quality chromatograms. Typically, the quality of the primer is not critical for the amplification of sequences of interest when the amplified PCR product is analyzed by agarose gel electrophoresis. On the contrary, when the primers are used for sequencing a template, the quality of the primers is critical for the sucess.

Mutations are commonly identified by direct sequencing of the PCR product. The primer quality is therefore even more critical when you want to detect or verify single base variants:

  • Genotyping by sequencing
  • Microsatellite analysis
  • Verification of Site-Directed Mutagenesis experiments

The standard sequencing primers shown below have the following predefined parameters:

Features of Standard PrimersDetails
Synthesis Scale50 nmol
Modificationsnone; only pre-defined sequences
Delivery FormatDry; in tubes

Available Lengths

15–30 (predefined)
Default PurificationHPSF (High-Purity Salt-Free)
Production days2 days*

*please add the shipping days to the production days to determined the delivery days.

Ordering

To order, simply select the checkbox for the predefined sequence below and hit add to cart. 

  Order Standard Primers

NameDNA sequence (5′→3′)LengthTemperature (°C)GC Content (%)PriceOrder
16FCGG TTA CCT TGT TAC GAC TT2055.245$8.50
16RAGA GTT TGA TCC TGG CTC AG2057.350$8.50
3AOXGCA AAT GGC ATT CTG ACA TCC2157.948$8.50
5AOXGAC TGG TTC CAA TTG ACA AGC2157.948$8.50
96gIIICCC TCA TAG TTA GCG TAA CG2057.350$8.50
BGHrevTAG AAG GCA CAG TCG AGG1856.056$8.50
CMVfCGC AAA TGG GCG GTA GGC GTG2165.767$8.50
CYC1ReverseGCG TGA ATG TAA GCG TGA C1956.753$8.50
EF-1alphaForTCA AGC CTC AGA CAG TGG TTC2159.852$8.50
EGFPC1FGAT CAC TCT CGG CAT GGA C1958.858$8.50
EGFPC1RCAT TTT ATG TTT CAG GTT CAG GG2357.139$8.50
EGFPN1FGTC GTA ACA ACT CCG CCC1858.261$8.50
EGFPN1RGTC CAG CTC GAC CAG GAT G1961.063$8.50
GadForGGG ATG TTT AAT ACC ACT AC2053.240$8.50
GadRevAAG AAA TTG AGA TGG TGC AC2053.240$8.50
Gal4ADTAC CAC TAC AAT GGA TG1747.941$8.50
Gal4BDTCA TCG GAA GAG AGT AG1750.447$8.50
GAPSEQFGTT CGA CCC CGC CTC GAT CC2065.570$8.50
GAPSEQRTGA CAC ACA TTC CAC AGG GTC2159.852$8.50
GL2CTT TAT GTT TTT GGC GTC TTC CA2357.139$8.50
ITS1TCC GTA GGT GAA CCT GCG G1961.063$8.50
ITS4TCC TCC GCT TAT TGA TAT GC2055.245$8.50
M13(-96)TGA GTT TCG TCA CCA GTA1851.444$8.50
M13FTGT AAA ACG ACG GCC AGT1853.750$8.50
M13F43AGG GTT TTC CCA GTC ACG ACG TT2362.452$8.50
M13RCAG GAA ACA GCT ATG ACC1853.750$8.50
M13R49GAG CGG ATA ACA ATT TCA CAC AGG2461.046$8.50
malEGGT CGT CAG ACT GTC GAT GAA GCC2466.158$8.50
mCherry-FCCC CGT AAT GCA GAA GAA GA2057.350$8.50
mCherry-RTTG GTC ACC TTC AGC TTG G1956.753$8.50
MycGCA TCA ATG CAG AAG CTG ATC TCA2461.046$8.50
pBABE3ACC CTA ACT GAC ACA CAT TCC2157.948$8.50
pBABE5CTT TAT CCA GCC CTC AC1752.853$8.50
pBabeFTGA CCT GGG AAG CCT TGG CT2061.460$8.50
pBabeRTTG CTG ACT AAT TGA GAT GCA TGC TTT2760.437$8.50
pJETSEQFCGA CTC ACT ATA GGG AGA GCG GC2366.061$8.50
pJETSEQRAAG AAC ATC GAT TTT CCA TGG CAG2459.342$8.50
pEXFGGA GCA GAC AAG CCC GTC AGG2165.767$8.50
pEXRCAG GCT TTA CAC TTT ATG CTT CCG GC2664.850$8.50
pBadFATG CCA TAG CAT TTT TAT CC2051.135$8.50
pBadRGAT TTA ATC TGT ATC AGG1846.933$8.50
pcDNAFGGC TAA CTA GAG AAC CCA CTG2159.852$8.50
pcDNARGGC AAC TAG AAG GCA CAG TC2059.355$8.50
petupATG CGT CCG GCG TAG A1654.363$8.50
pFBACfTCC GGA TTA TTC ATA CCG TCC C2260.350$8.50
pFBACrCCT CTA CAA ATG TGG TAT GGC TG2360.648$8.50
pGexFATA GCA TGG CCT TTG CAG G1956.753$8.50
pGexRGAG CTG CAT GTG TCA GAG G1958.858$8.50
pGL3forCTA GCA AAA TAG GCT GTC CC2057.350$8.50
pGLforGTA TCT TAT GGT ACT GTA ACT G2254.736$8.50
pGLrevCTT TAT GTT TTT GGC GTC TTC C2256.541$8.50
pJET12FCGA CTC ACT ATA GGG AGA GCG GC2366.061$8.50
pJET12RAAG AAC ATC GAT TTT CCA TGG CAG2459.342$8.50
pJET1FGCC TGA ACA CCA TAT CCA TCC2159.852$8.50
pJET1RGCA GCT GAG AAT ATT GTA GGA GAT C2561.344$8.50
pQEforGTA TCA CGA GGC CCT TTC GTC T2262.155$8.50
pQErevCAT TAC TGG ATC TAT CAA CAG GAG2459.342$8.50
pShCMVfGGT CTA TAT AAG CAG AGC TG2055.245$8.50
pShCMVrGTG GTA TGG CTG ATT ATG ATC AG2358.944$8.50
RV3CTA GCA AAA TAG GCT GTC CCC2159.852$8.50
SeqL-ATCG CGT TAA CGC TAG CAT GGA TCT C2564.652$8.50
SeqL-BGTA ACA TCA GAG ATT TTG AGA CAC2457.638$8.50
SP6CAT TTA GGT GAC ACT ATA G1950.237$8.50
SV40pA-RGAA ATT TGT GAT GCT ATT GC2051.135$8.50
SV40-promoterTAT TTA TGC AGA GGC CGA GG2057.350$8.50
T3AAT TAA CCC TCA CTA AAG GG2053.240$8.50
T7TAA TAC GAC TCA CTA TAG GG2053.240$8.50
T7EEVATG TCG TAA TAA CCC CGC CCC G2264.059$8.50
T7PCS2TGT CTG GAT CTA CGT AAT ACG2155.943$8.50
T7termCTA GTT ATT GCT CAG CGG T1954.547$8.50
TrcHisFAAT CTG TGT GGG CAC TCG1856.056$8.50
TrcHisRCTT CTG CGT TCT GAT TTA ATC TG2357.139$8.50
V1-5GGA CTT TCC AAA ATG TCG1851.444$8.50
V5ACC GAG GAG AGG GTT AGG GA2061.460$8.50
VF2TGC CAC CTG ACG TCT AAG AA2057.350$8.50
VRATT ACC GCC TTT GAG TGA GC2057.350$8.50
XL39ATT AGG ACA AGG CTG GTG GG2059.355$8.50