Dual Labeled Probes suitable for all type of qPCR instruments

Dual labeled probes used in quantitative real-time PCR systems take advantage of the 5'→3' exonuclease activity of Taq polymerase.

Dual labeled probes contain a fluorescent reporter and the appropriate quencher covalently linked to the 5′ and 3′ termini, respectively, of a custom oligo sequence which anneals with the target DNA between the PCR primers. During the extension phase of PCR, the 5'→3' exonuclease activity of Taq polymerase cleaves the fluorescent reporter from the probe. The amount of free reporter accumulates as the number of PCR cycles increases. The fluorescent signal from the free reporter is measured in real time and allows the quantification of the target sequence.

Ordering

Eurofins Genomics offers the most popular dye-quencher combinations with predefined probe pricing. More dye-quencher combinations are available from the custom oligo order page. View a list of all modifications for custom probes.

 Pricing is located on the price list page, under modifications.

Product specifications:

• Degenerate bases (wobbles) included
• Multiple Dye–Quencher combinations (see below)
• Shipped after HPLC purification
• Quality control by OD measurement and MALDI-TOF MS
• Delivery: lyophilized or concentration adjusted in tubes
• Shipped in 4–5 working days

All relevant documents are provided in your online account free of charge:

• Oligo synthesis report
• Quality report including MALDI-TOF MS spectra
• Delivery note

1. The synthesis scale indicates the initial amount of 3'-bases.
2. Average yield was determined for a 20-mer; Calculation: 1 OD = 5 nmole = 30 ug; may vary for sequences with GC content >70%, >3 purine stretches, or strong secondary structures.

Probe Combinations