Dual Labeled Probes with locked nucleic acids
LocNA probes and primers contain Locked Nucleic Acid bases which improves the stability and performance of qPCR assays. These modified bases provide a 2'-O, 4'-C methylene bridge which effectively "locks" the 2' oxygen to the 4' carbon. LocNA probes and primers are the optimal choice when designing highly-specific, shorter probes with challenging target sequences. This novel approach has opened up a broad range of possibilities for researchers working in qPCR.
Benefits
- Greater stability in assays.
- Higher performance in assays.
- Allows for more flexibility and possibilities when working on challenging target sequences.
- Especially effective in target regions with a low GC percentage, AKA shorter target sequences.
- Enhanced accuracy, SNP, and hybridization.
Specifications
- Sequence length from 5 - 40 bases
- Up to 15 LocNA bases per probe are possible
- Only DNA bases are allowed at the 5' and 3' end
- HPLC purified by default
- Delivered lyophilized (dry down) or concentration adjusted in tubes
- Production takes 5 working days
- QC report incl. ESI
- Average synthesis yields of 15 nmole resp. 2 OD
Ordering Options
Clink the links below to visit the LocNA order page for either probes or primers.
Order
Order LocNA primers
Available dye-quencher combinations:
*Contact us for custom probe projects not shown below.
5' Reporter |
Abs [nm] |
Em [nm] |
3' Quencher |
FAM [FAM]
|
495 |
520 |
TAM, BHQ1, TQ2 |
TET [5TET]
|
521 |
536 |
TAM, BHQ1 |
JOE [JOE]
|
520 |
548 |
TAM, BHQ1, TQ2 |
HEX [5HEX]
|
535 |
556 |
BHQ1, BHQ2, TQ2, TQ3 |
Cyanine3 [Cy3]
|
552 |
570 |
BHQ1, BHQ2 |
ROX [ROX]
|
575 |
602 |
BHQ2, TQ3 |
Texas Red [TxRed]
|
583 |
603 |
BHQ2, TQ3 |
Cal Fluor 590 [CalFluor590]
|
569 |
606 |
BHQ2 |
Cal Fluor Red 610 [CalFluor610]
|
590 |
610 |
BHQ2 |
ATTO 647N [ATTO647N]
|
644 |
669 |
BHQ2 |
Cyanine5 [Cy5]
|
649 |
670 |
BHQ2, TQ3 |
Additional Probe Options