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Standard Primers

One click ordering for universal primers

In order to enable fast and convenient ordering of sequencing primers that are widely used to sequence inserts in standard cloning vectors, we have assembled a list of primer sequences that you can order with a single click. Simply select the primer and add it to the cart. We have also tailored the post-synthesis work flow to ensure that these primers have the quality that will provide the best sequencing results.

Typically, the quality of the primer is not critical for the amplification of sequences of interest when the amplified PCR product is analyzed by agarose gel electrophoresis. On the contrary, when the primers are used for sequencing a template, the quality of the primers is critical for the sucess.

Mutations are commonly identified by direct sequencing of the PCR product. The primer quality is therefore even more critical when you want to detect or verify single base variants:

  • Genotyping by sequencing
  • Microsatellite analysis
  • Verification of Site-Directed Mutagenesis experiments

The standard sequencing primers ordered via the Standard Sequencing primer have the following predefined parameters:

Features of Standard Primers Details
Synthesis Scale 50 nmol
Modifications none; only pre-defined sequences
Delivery Format Dry; in tubes
Available Lengths  15–30 (predefined)
Default Purification HPSF (High-Purity Salt-Free)
Production days 2 days*

*please add the shipping days to the production days to determined the delivery days.