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ELISA Test Kits

SARS-CoV-2 IgG, IgA and IgM ELISAs are intended for the qualitative detection of IgG, IgA and IgM antibodies to SARS-CoV-2 virus in human serum to aid in the diagnosis of Coronavirus (COVID-19). The assays are an ideal supplement for direct pathogen detection and are a valuable tool for epidemiological studies.

The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter plates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labeled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step, unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint color. Absorbance at 450/620 nm is read using an ELISA Microtiter plate reader.

 

Test principle: Enzyme-linked Immunosorbent Assay

Format: Microtiter plate with 96 wells

Incubation Time: 2 hours

Shelf Life (months):  6

Storage:   2–8°C

 

GSD SARS-CoV-2 ELISA IgA

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GSD SARS-CoV-2 ELISA IgA

The GSD SARS-CoV-2 IgG, IgA and IgM ELISAs are intended for the qualitative detection of IgG, IgA and IgM antibodies to SARS-CoV-2 virus in human serum to aid in the diagnosis of Coronavirus (COVID-19). The assays are an ideal supplement for direct pathogen detection and are a valuable tool for epidemiological studies.


Assay Features

  • Separate Detection of IgG, IgM and IgA Antibodies
  • Advantages of our Reagent System
        ∙ Total incubation under 2 hours
         Ready to use controls
         Assay procedure is easy to automate


Assay Specifications

Article nr:     GSD01-1029
Nr. of Reactions   96
Sample matrix:  Serum
Dilution:    1:101
Incubation time:  30min/30min/30min
Incubation Temperature: Room temperature
Storage 2-8 °C

                                

Reagents supplied

  • 96 well breakable microtiter plate, coated with SARS-CoV-2 antigen, stabilized in a foil pouch, ready to use.
  • Dilution Buffer, 2 x 50ml, with preservative and Tween 20, ready to use.
  • Wash Concentrate 20X, 50ml, with preservative and Tween 20.  Dilute with 1000ml distilled or deionized water before use.
  • Negative Control, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
  • Calibrator, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
  • Positive Control, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
  • Conjugate IgA 13.5ml, goat anti-human IgA antibodies with protein stabilizer and preservative, ready to use.
  • Substrate Solution (TMB), 13.5ml, ready to use.
  • Stop Solution, 7.5ml, ready to use.

GSD SARS-CoV-2 ELISA IgM

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GSD SARS-CoV-2 ELISA IgM

The GSD SARS-CoV-2 IgG, IgA and IgM ELISAs are intended for the qualitative detection of IgG, IgA and IgM antibodies to SARS-CoV-2 virus in human serum to aid in the diagnosis of Coronavirus (COVID-19). The assays are an ideal supplement for direct pathogen detection and are a valuable tool for epidemiological studies.


Assay Features

  • Separate Detection of IgG, IgM and IgA Antibodies
  • Advantages of our Reagent System
        ∙ Total incubation under 2 hours
         Ready to use controls
         Assay procedure is easy to automate


Assay Specifications

Article nr:     GSD01-1030
Nr. of Reactions   96
Sample matrix:  Serum
Dilution:    1:101
Incubation time:  30min/30min/30min
Incubation Temperature: Room temperature
Storage 2-8 °C

                                

Reagents supplied

  • 96 well breakable microtiter plate, coated with SARS-CoV-2 antigen, stabilized in a foil pouch, ready to use.
  • Dilution Buffer, 2 x 50ml, with preservative and Tween 20, ready to use.
  • Wash Concentrate 20X, 50ml, with preservative and Tween 20.  Dilute with 1000ml distilled or deionized water before use.
  • Negative Control, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
  • Calibrator, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
  • Positive Control, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
  • Conjugate IgM 13.5ml, goat anti-human IgM antibodies with protein stabilizer and preservative, ready to use.
  • Substrate Solution (TMB), 13.5ml, ready to use.
  • Stop Solution, 7.5ml, ready to use.
  • RF-Adsorption solution, 4.5ml, ready to use.

GSD SARS-CoV-2 ELISA IgG

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GSD SARS-CoV-2 ELISA IgG

The GSD SARS-CoV-2 IgG, IgA and IgM ELISAs are intended for the qualitative detection of IgG, IgA and IgM antibodies to SARS-CoV-2 virus in human serum to aid in the diagnosis of Coronavirus (COVID-19). The assays are an ideal supplement for direct pathogen detection and are a valuable tool for epidemiological studies.


Assay Features

  • Separate Detection of IgG, IgM and IgA Antibodies
  • Advantages of our Reagent System
        ∙ Total incubation under 2 hours
         Ready to use controls
         Assay procedure is easy to automate


Assay Specifications

Article nr:     GSD01-1028
Nr. of Reactions   96
Sample matrix:  Serum
Dilution:    1:101
Incubation time:  30min/30min/30min
Incubation Temperature: Room temperature
Storage 2-8 °C

                                

Reagents supplied

  • 96 well breakable microtiter plate, coated with SARS-CoV-2 antigen, stabilized in a foil pouch, ready to use.
  • Dilution Buffer, 2 x 50ml, with preservative and Tween 20, ready to use.
  • Wash Concentrate 20X, 50ml, with preservative and Tween 20.  Dilute with 1000ml distilled or deionized water before use.
  • Negative Control, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
  • Calibrator, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
  • Positive Control, 1.5ml, human serum with protein-stabilizer and preservative, ready to use.
  • Conjugate IgG 13.5ml, goat anti-human IgM antibodies with protein stabilizer and preservative, ready to use.
  • Substrate Solution (TMB), 13.5ml, ready to use.
  • Stop Solution, 7.5ml, ready to use.