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Eurofins Genomics can provide the primer and probe sequences recommended by the CDC to researchers working on the coronavirus. The sequences were publically provided by the CDC here. Researchers can order these primers and probes to create a panel in their lab, following the panel instructions from the CDC. These materials are provided for research purposes only. Eurofins Genomics does not claim that these materials are in any way endorsed or validated by the CDC.

Ordering

There are multiple ways to order the CDC sequences but the easiest is to use the custom DNA oligo order page since it accommodates both modified probes and primers. These materials are for research use only.

Custom DNA Order Page

Gene Order Page

Gene Fragments

Alternative Ordering Methods

CDC Approved Sequences

*These sequences can be entered on the custom DNA oligo tube order page.

2019-Novel Coronavirus (2019-nCoV) Real-time rRT-PCR Panel Primers and Probes

Name

Description

Oligonucleotide Sequence (5’>3’)

Label1

Working

Conc.

2019-nCoV_N1-F

2019-nCoV_N1

Forward Primer

5’-GAC CCC AAA ATC AGC GAA AT-3’

None

20 µM

2019-nCoV_N1-R

2019-nCoV_N1

Reverse Primer

5’-TCT GGT TAC TGC CAG TTG AAT CTG-3’

None

20 µM

2019-nCoV_N1-P

2019-nCoV_N1

Probe

5’-FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1-3’

FAM, BHQ-1

5 µM

2019-nCoV_N2-F

2019-nCoV_N2

Forward Primer

5’-TTA CAA ACA TTG GCC GCA AA-3’

None

20 µM

2019-nCoV_N2-R

2019-nCoV_N2

Reverse Primer

5’-GCG CGA CAT TCC GAA GAA-3’

None

20 µM

2019-nCoV_N2-P

2019-nCoV_N2

Probe

5’-FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1-3’

FAM, BHQ-1

5 µM

2019-nCoV_N3-F

2019-nCoV_N3

Forward Primer

5’-GGG AGC CTT GAA TAC ACC AAA A-3’

None

20 µM

2019-nCoV_N3-R

2019-nCoV_N3

Reverse Primer

5’-TGT AGC ACG ATT GCA GCA TTG-3’

None

20 µM

2019-nCoV_N3-P

2019-nCoV_N3

Probe

5’-FAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ1-3’

FAM, BHQ-1

5 µM

RP-F

RNAse P Forward

Primer

5’-AGA TTT GGA CCT GCG AGC G-3’

None

20 µM

RP-R

RNAse P Reverse

Primer

5’-GAG CGG CTG TCT CCA CAA GT-3’

None

20 µM

RP-P

RNAse P

Probe

5’-FAM – TTC TGA CCT GAA GGC TCT GCG CG – BHQ-1-3’

FAM, BHQ-1

5 µM

           

Note: Oligonucleotide sequences are subject to future changes as the 2019-Novel Coronavirus evolves.

Publications

  1. (2020) 2019 Novel Coronavirus (2019-nCoV), Wuhan, China. [Online] US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
  2. (2020) 2019-Novel coronavirus (2019-nCoV) real-time rRT-PCR panel primers and probes. US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
  3. (2020) Real-time RT-PCR panel for detection 2019-novel coronavirus. US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].
  4. (2020) Emergency use authorization. [Online] US Centers for Disease Control and Prevention. [Accessed 28 Jan 2020].

 

CDC Information for Laboratories

Eurofins Genomics is not, and does not claim to be, a primary, preferred or recommended source of the CDC coronavirus primer-probe panel.  Eurofins Genomics is independently offering the sequences that were published by the CDC for use in coronavirus detection. These materials are for research use only. They have not been designed, tested, validated by CDC, nor are they in any way endorsed by the CDC or any other public health agency.

Panel Instructions from CDC

CLICK HERE to read the instructions for creating a panel, as provided by the CDC.