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Transcriptome Sequencing

Dig deeper into the expressed genes and gene regulation mechanisms

Next generation sequencing to shed a new light on your transcriptome and regulome studies.

Depending on your needs there are three different services available for transcriptome sequencing projects. A specific set of libraries is available for each section, offering data generation according to your needs.

 

De Novo Transcriptome Sequencing »

mRNA-Seq libraries for sequencing on Illumina HiSeq 2500

Find out which genes are expressed at a given time and at a given physiological background

 

Expression Profiling »

mRNA-Seq libraries

Compare expression levels of transcripts from different samples (quantitative analysis)

 

Small RNA Sequencing »

Small RNA-Seq library

Identify novel small RNAs or target molecules of novel miRNAs. Characterise small RNA expression from different samples

 

Need more information for your specific project or an individual quote? Just contact us!

Selected publications about transcriptome sequencing services

2016

Microminipig liver transcriptomes: Sakai, C. et al., Transcriptome of the quorum-sensing signal-degrading Rhodococcus erythropolis responds differentially to virulent and avirulent Pectobacterium atrosepticum. DOI: 10.1002/bdd.2007

 

2015

Expression Profiling (HiSeq): Kwasiborski, A. et al., Transcriptome of the quorum-sensing signal-degrading Rhodococcus erythropolis responds differentially to virulent and avirulent Pectobacterium atrosepticum. Heredity

 

2014

Expression profiling (HiSeq): Boenigk, J. et al., Effects of silver nitrate and silver nanoparticles on a planktonic community: general trends after short-term exposure. PloS one 9(4)

De novo transcriptome analysis: Højland, D. et al., Adaptation of Musca domestica L. field population to laboratory breeding causes transcriptional alterations. PloS one 9(1)

De novo transcriptome analysis: Merlot, S. et al., The metal transporter PgIREG1 from the hyperaccumulator Psychotria gabriellae is a candidate gene for nickel tolerance and accumulation. Journal of experimental botany

De novo transcriptome with FLX: Safavi-Hemami et al. Diversity of Conotoxin Gene Superfamilies in the Venomous Snail, Conus victoriae. PLoS ONE 9(2)

De novo transcriptome with FLX: Yamauchi et al. Newly Developed SNP-Based Identification Method of Hop Varieties. ASBC 72(4)

Expression profiling: Amich et al. Regulation of sulphur assimilation is essential for virulence and affects iron homeostasis of the human-pathogenic mould Aspergillus fumigatus. PLOS One, 9(8)

De novo transcriptome analysis FLX & gene expression HiSeq: Shimizu et al. Qualitative de novo analysis of full length cDNA and quantitative analysis of gene expression for common marmoset (Callithrix jacchus) transcriptomes using parallel long-read technology and short-read sequencing. PLOS One, 9(6)

 

2013

Small RNA sequencing: Bizuayehu et al. Characterization of Novel Precursor miRNAs Using Next Generation Sequencing and Prediction of miRNA Targets in Atlantic Halibut. PLOS ONE 8(4)

De novo transcriptome with FLX: D'Agostino et al. Genomic analysis of the native European Solanum species, S. dulcamara. BMC Genomics 14:356

Expression profiling: Gould et al. Deep sequencing of Trichomonas vaginalis during the early infection of vaginal epithelial cells and amoeboid transition. International Journal for Parasitology [Epub ahead of print]

De novo transcriptome with FLX: Passos M et al. Analysis of the leaf transcriptome of Musa acuminata during interaction with Mycospharella musicola: gene assembly, annotation and marker development. BMC Genomics 14:78

 

2012

De novo transcriptome with FLX: Urbarova I et al. Digital Marine Bioprospecting: Mining new neurotoxin drug candidates from the transcriptomes of cold-water sea anemones. Marine Drugs 10(10)

De novo transcriptome with HiSeq: Mutasa-Göttgens E et al. A new RNASeq-based reference transcriptome for sugar beet and its application in transcriptome-scale analysis of vernalization and gibberellin responses. BMC Genomics 13:99

De novo transcriptome with FLX: Hroudova M et al. Analysis of gene expression for microminipig liver transcriptomes using parallel long-read technology and short-read sequencing. PLoS ONE 7(4):e36420.

 

2011

De novo transcriptome with FLX: Windisch H et al. Thermal acclimation in Antartic fish: transcriptomic profiling of metabolic pathways. AJP - Regu Physiol 301, 5

De novo transcriptome with FLX: Kuzina V, et al. Barbarea vulgaris linkage map and quantitative trait loci for saponins, glucosinolates, hairiness and resistance to the herbivore Phyllotreta nemorum. Phytochemistry. 2011 Feb 72, 2-3

 

2010

De novo transcriptome with FLX: Young, N.D. et al. Elucidating the transcriptome of Fasciola hepatica – A key to fundamental and biotechnological discoveries for a neglected parasite. Biotechnology Advances (2010) 28, 222-231

De novo transcriptome with FLX: Elmer KR. et al. Rapid sympatric ecological differentiation of crater lake cichlid fishes within historic times. BMC Biol. 2010 May 12; 8:60

De novo transcriptome with FLX: Johansen SD. et al. Approaching marine bioprospecting in hexacorals by RNA deep sequencing. New Biotechnology, Vol 27

 

2008

Expression profiling: Torres, T.T., Ottenwälder, B. et al. Gene expression profiling by massively parallel sequencing. Genome Research (2008) 18: 172-177


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