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New updates to the Oligo portfolio

In the genomics era, where the unlimited possibilities of discovering new genetic frontiers are limited only by your budget and time, we have made enhancements to our oligo portfolio to allow you to reach a little further faster and within your budget and time. Don't sacrifice speed or quality in your pursuit of groundbreaking science.

Express Oligos: Express service at regular price for your primers

Finally, a service that is in step with the pace of your research and within your budget. Order Express Oligos at the price of regular oligos as late as 5:00 pm EST and have them delivered next day with Overnight Shipping. Don't let high per-base price of synthesis or other handling charges make a dent in your tight budget and slow down your work.
To learn more about this service, click here.

High-quality low-yield oligos in plates (4 nmol synthesis scale)

Why order boatloads of oligos when they will be used only a few times and gather ice in your freezer. Order oligos synthesized at 4 nmol synthesis scale in 96-well plates and save on your oligo budget. These oligos are synthesized on our new synthesis platform. Use them in your application to test their performance.
To learn more about this product, click here.

Get your oligos from the pioneer of high-throughput oligo synthesis

When you order a custom DNA oligo from us, you get:

  • Easy online ordering interface
  • Single stranded custom DNA*
  • Synthesis scales from 0.01 μmol to 10 μmol
  • Huge variety of available modifications
  • Different purification options to choose from
  • Delivered lyophilized or hydrated (normalized)
  • Shipped in tubes or 96-well plates

High Quality Oligos. Fast Turn-Around Times
With a view to ensure that your research is not delayed due to the delivery of reagents that function as expected, our custom DNA oligos are:

  • Synthesized using highly efficient phosphoramidite chemistry
  • Synthesized in a high-throughput production facility
  • Processed using highly automated technologies
  • Assayed by MALDI-TOF MS analyses and OD measurement
  • Monitored by a sophisticated barcode driven system

*To order linear, double-stranded DNA oligos, please visit the GeneStrands gene fragments page here.

Wide range of synthesis scales, purification options and turnaround time

We perform MALDI-TOF MS analysis on every custom DNA Oligo; infact we pioneered 100% MS testing in 1998. You can place an order for one or several oligos. For orders with large number of oligos, we recommend that you have the oligos delivered in plates.

Features Oligos in Tubes Oligos in Plates
Synthesis Scales (in nmoles) 10, 25, 50, 200, 1000, & 10,000 4, 10, 25, 50, & 200
Modifications Several Fewer
Degenerate bases (see below) Single letter IUB code and predefined compositions
Available Oligo Lengths (in nt) 5–125
Synthesis Chemistry β-cyanoethyl Phosphoramidite coupling
Default Purification Salt-free purification
Other Purification Options HPSF, HPLC, PAGE, 2-Step HPSF, HPLC, PAGE
Delivery Formats Lyophilized or in solution (normalized) Lyophilized or in solution (normalized)

 

Typical Turnaround Times for Standard Custom Oligos in Tubes

Synthesis Scale (nmol) Purification Delivered in (days)
10 nmol (Length: 5–60 nt)
or
25 nmol (Length: 5–80 nt)
Salt-Free 1
HPSF 2
HPLC NA
PAGE NA
50 nmol (Length: 5–90 nt) Salt-Free 1
HPSF 2
HPLC 5
PAGE 7
200 nmol (Length: 5–125 nt)
or
1,000 nmol (length: 5–125 nt)
Salt-Free 2
HPSF 5
HPLC 5
PAGE 7
10,000 nmol (Length: 5–100 nt) Salt-Free 3
HPSF NA
HPLC 5
PAGE NA

Typical Turnaround Times for standard Custom Oligos in Plates

Synthesis Scale (available lengths) Purification Delivered in (days)
4 nmol (Length: 5–60 nt) Salt-Free 3

10 nmol (Length: 5–60 nt)
or
25 nmol (Length: 5–80 nt)

Salt-Free 3
HPSF 3
PAGE NA
50 nmol (Length: 5–90 nt) Salt-Free 3
HPSF 3
PAGE Inquire
200 nmol (Length: 5–125 nt) Salt-Free 3
HPSF 3
PAGE Inquire 
1,000 nmole (Length: 5–125 nt)
or
higher
Salt-Free Inquire
HPSF Inquire
PAGE Inquire

 

Standard IUPAC/IUB single letter codes for degenerate bases
Degenerate bases in primers serve a useful purpose in amplifying homologous genes from other species; moreso when the amino acid sequence of the homologoes protein from the species is known. The presence of degeneracy in the bases of the primer sequence accomodates the potential difference in the genetic sequence and yeilds an amplified product. To order oligos with such degenerate bases in the sequence, include the single letter code for the base as recognized by IUPAC and IUB to incorporate equal quantities of each base represented by that letter at the desired positions.

Single Letter Code Represents
B C, G, T (not A)
D A, G, T (Not C)
H A, C, T (Not G)
K T, G (Keto)
M A, C (Amino)
N A, C, G, T (Any base)
Single Letter Code Represents
R A, G (Purine)
S G, C (Strong)
W A, T (Weak)
V A, C, G (Not T)
Y C, T (Pyrimidine)

Custom DNA oligos at Eurofins Genomics are synthesized by coupling the relevant series of β-cyanoethyl phorphoramidites on pre-packed controlled-pore solid supports before being deprotected, de-salted, quantitated and being subject to quality control assessment. Typical coupling efficiencies acheived using our automated synthesis platforms are grater than 99%. The coupling efficiencies, when coupled with the length of the oligo sequence to be synthesized, essentially determine the yield of the oligo. Eurofins Genomics has optimized its synthesis scale and sequence length using its traditional high-throughput synthesis platforms to produce high-quality oligos of different lengths.

Lengths of oligonucleotides available in tubes and plates at different synthesis scales

Sythesis Scale (nmol) Available Lengths (in nts)
10 5–60
25 5–80
50 5–90
200 5–125
1,000 5–125
10,000 5–100

 

Purification options available for oligos in tubes and plates based on the length of the oligo and the synthesis scale:

Purification Option Purity Level Range of Oligo Length (in nts) Synthesis Scales (in nmols)
Salt-Free na 5–125 4*, 10, 25, 50, 200, 1000, 10000
HPSF* Reversed-phase cartridge 10–100 10, 25, 50, 200, 1000,
HPLC >85% 5–100 50, 200
HPLC 2-Step >90% 5–125 200, 1000, 10000
PAGE ˜90% 20–125 50, 200
PAGE 2-Step >90% 20–125 200, 1000

*Oligos synthesized at the 4 nmol scale are available only for delivery in 96-well plates. 

Please note that some oligos, particularly those containing modifications, are offered only with specific purification options. To see the yield of standard unmodified oligos based on the purification and length of the oligo, please visit the yields and purifications page.  

cGMP Oligos, Mixing and Pooling and other custom Services

Besides having the oligos purified by our purification specialists, we offer additional services based on the type of oligo(s) ordered or the intended application.

Overnight/Express Oligos

Refinements in oligo production processes, when coupled with our location being just a few miles from the UPS office in Louisville, now allows us to offer Express OligosTM—you can plan your routine PCR experiments today and run the experiment tomorrow without having to wait for the delivery of primer for two days or pay a premium for the expedited shipping.

Mixing & Pooling

Applications such as site directed mutagenesis, gene amplification, or other gene expression assays require the use of equimolar or pre-defined concentrations of two or more oligos. Our Production team offers a wide variety of customization options, including delivery of oligos in specific tubes or plates. Oligo panels can also be prepared utilizing our ISO-certified lab procedures to ensure batch to batch reproducibility. Please submit the Oligo Mixing and Pooling Inquiry form if you’d like more details and/or a quote. Our Special Projects representative will contact you with additional details.

cGMP oligos

Our DNA Synthesis lab at Louisville is equipped to synthesized oligos with customized requirements, lot-to-lot reproduceability and full-process traceability. To learn more about the benefits of oligos manufactured using cGMP, please visit the cGMP Oligonucleotides page.

 

At Eurofins Genomics, we recognize that your research is valuable. To ensure that the oligos synthesized at our facilities meet all the requirements of your experimental application we have streamline the production process and implemented several quality controls at different stages of the production process. The quality control checks implemented at various points during the production process are displayed below:

Custom -dna -quality

Production process

The integration of the ecommerce site with the back-end laboratory information managment systems (LIMS) ensures that each synthesized oligo is tracked through the various steps in the production process. As soon as an oligo order is placed on the ecom site, the automated order confirmation email is sent and the details of the order is transferred to the production lab without any loss in the order data. An unique barcode assigned to each oligo order ensures that the production status of an oligo can be monitored at any given instance.

Quality Control Processes

Quality controls processes are designed to evalaute both individual oligos or the total process.

Quality Control for Oligos

Every oligo is assayed using mass spectra. Shorter oligos (≤50 nts long) are typically evalauted using Matrix-Assisted Laser Desorption Ionization (MALDI) Time-of-Flight (TOF) mass spectrometry, while the longer oligos (≥50 nts) are analyzed by Electro-Spray Ionization (ESI) mass spectrometry. For oligos used in special applications, e.g., qPCR probes, purification and analysis is carried out using HPLC.

Quality Control for Production Processes

To assess the whole production process flow, orders for production control primers and probes are placed randomly each day. These orders proceed through the work-flow anonymously and are evaluated in qPCR reactions or assessed by our Quality Assurance team.