Standard Primer Sequence Data

Over the years, our scientists have sequenced several templates using the primer sequences below. These primers will added to your samples for no extra charge when indicated in your sequencing orders. We also encourage you to order these primers and prepare your 'premixed' samples. Amongst the different types of samples sequenced (excluding samples in ready2load plates), the premixed samples that require standard sequencing chemistry are processed at the most rapid pace and the sequence data is released earliest. To order any of the primers listed here, please use the Standard Primers link to avail a one-click ordering protocol for a HPSF-purified sequencing grade primer. The delivered primer can be used in over >500 sequencing reactions.

 Primer Name DNA Sequence (5′→3′)
16F CGG TTA CCT TGT TAC GAC TT
16R AGA GTT TGA TCC TGG CTC AG
3AOX GCA AAT GGC ATT CTG ACA TCC
5AOX GAC TGG TTC CAA TTG ACA AGC
96gIII CCC TCA TAG TTA GCG TAA CG
BGHrev TAG AAG GCA CAG TCG AGG
CMVf CGC AAA TGG GCG GTA GGC GTG
CYC1Reverse GCG TGA ATG TAA GCG TGA C
EF-1aForward TCA AGC CTC AGA CAG TGG TTC
EGFPC1F GAT CAC TCT CGG CAT GGA C
EGFPC1R CAT TTT ATG TTT CAG GTT CAG GG
EGFPN1F GTC GTA ACA ACT CCG CCC
EGFPN1R GTC CAG CTC GAC CAG GAT G
GadFor GGG ATG TTT AAT CCA CTA C
GadRev AAG AAA TTG AGA TGG TGC AC
Gal4AD TAC CAC TAC AAT GGA TG
Gal4BD TCA TCG GAA GAG AGT AG
GAPSEQF GTT CGA CCC CGC CTC GAT CC
GAPSEQR TGA CAC ACA TTC CAC AGG GTC
GL2 CTT TAT GTT TTT GGC GTC TTC CA
ITS1 TCC GTA GGT GAA CCT GCG G
ITS4 TCC TCC GCT TAT TGA TAT GC
M1396 TGA GTT TCG TCA CCA GTA
M13F TGT AAA ACG ACG GCC AGT
M13F43 AGG GTT TTC CCA GTC ACG ACG TT
M13R CAG GAA ACA GCT ATG ACC
M13R49 GAG CGG ATA ACA ATT TCA CAC AGG
malE GGT CGT CAG ACT GTC GAT GAA GCC
mCherry-F CCC CGT AAT GCA GAA GAA GA
mCherry-R TTG GTC ACC TTC AGC TTG G
Myc GCA TCA ATG CAG AAG CTG ATC TCA
pBabe3 ACC CTA ACT GAC ACA CAT TCC
pBabe5 CTT TAT CCA GCC CTC AC
pBabeF TGA CCT GGG AAG CCT TGG CT
pBabeR TTG CTG ACT AAT TGA GAT GCA TGC TTT
pBadF ATG CCA TAG CAT TTT TAT CC
pBadR GAT TTA ATC TGT ATC AGG
pcDNAF GGC TAA CTA GAG AAC CCA CTG
pcDNAR GGC AAC TAG AAG GCA CAG TC
petup ATG CGT CCG GCG TAG A
pexF GGA GCA GAC AAG CCC GTC AGG
pexR CAG GCT TTA CAC TTT ATG CTT CCG GC
pFBACf TCC GGA TTA TTC ATA CCG TCC C
pFBACr CCT CTA CAA ATG TGG TAT GGC TG
pGexF ATA GCA TGG CCT TTG CAG G
pGexR GAG CTG CAT GTG TCA GAG G
pGL3for CTA GCA AAA TAG GCT GTC CC
pGLfor GTA TCT TAT GGT ACT GTA ACT G
pGLrev CTT TAT GTT TTT GGC GTC TTC C
pJET12F CGA CTC ACT ATA GGG AGA GCG GC
pJET12R AAG AAC ATC GAT TTT CCA TGG CAG
pJET1F GCC TGA ACA CCA TAT CCA TCC
pJET1R GCA GCT GAG AAT ATT GTA GGA GAT C
pJETSEQF CGA CTC ACT ATA GGG AGA GCG GC
pJETSEQR AAG AAC ATC GAT TTT CCA TGG CAG
pQEfPR GTA TCA CGA GGC CCT TTC GTC T
pQErev CAT TAC TGG ATC TAT CAA CAG GAG
pShCMVf GGT CTA TAT AAG CAG AGC TG
pShCMVr GTG GTA TGG CTG ATT ATG ATC AG
RV3 CTA GCA AAA TAG GCT GTC CCC
SeqL-A TCG CGT TAA CGC TAG CAT GGA TCT C
SeqL-B GTA ACA TCA GAG ATT TTG AGA CAC
SP6 CAT TTA GGT GAC ACT ATA G
SV40pA-R GAA ATT TGT GAT GCT ATT GC
SV40-promoter TAT TTA TGC AGA GGC CGA GG
T3 AAT TAA CCC TCA CTA AAG GG
T7 TAA TAC GAC TCA CTA TAG GG
T7EEV ATG TCG TAA TAA CCC CGC CCC G
T7PCS2 TGT CTG GAT CTA CGT AAT ACG
T7term CTA GTT ATT GCT CAG CGG T
TrcHisF AAT CTG TGT GGG CAC TCG
TrcHisR CTT CTG CGT TCT GAT TTA ATC TG
V1-5 GGA CTT TCC AAA ATG TCG
V5 ACC GAG GAG AGG GTT AGG GA
VF2 TGC CAC CTG ACG TCT AAG AA
VR ATT ACC GCC TTT GAG TGA GC
XL39 ATT AGG ACA AGG CTG GTG GG