Article page image

SimpleSeq wraps all your Sanger sequencing needs into a convenient little package. Each SimpleSeqTM kit includes the sequencing service for 96 sequencing reactions; so you can submit samples for all 96 sequencing reactions at once or submit as low as one sample at a time. The sequencing results will be available for download by noon the day after shipping the sample.

Important Update!

New System

 New -flow

 

Old System

Overflow -Old -system -v 2c

 Overflow -Graphic -Legend

Issues with Old Process Benefits of New Process
  • When the first SimpleSeq kit is exhausted (0 reactions), it takes reactions from another kit.
  • User must buy more SimpleSeq kits just to use leftover tubes in the first SimpleSeq kit.
  • This creates more and more leftover tubes
  • No way to separate kits.  Shared accounts can accidentally take a reaction from another colleague’s kit.
  • Little traceability between who is using each tube and reaction in a kit.
  • Must order a new kit for new reactions, or call customer support to “reactivate” a tube.  Both options take time.
  • More kits means more leftover tubes. The problem only grows worse over time.
  • None of your existing reactions are lost! If you have available reactions in your account, you can submit sequencing orders at no charge, exactly as before.  Please see the section below for details.
  • Easy to share kits.
  • Full visibility and traceability for all users sharing a kit.
  • FlexReads can be used with any kit, no restrictions.
  • No more leftover tubes!

 

Trade-in Program

Want to trade in your existing SimpleSeq kits for FlexReads?  Please email us if interested.

The trade-in option is good for customers that purchased a large number of kits only for the reactions, not the tubes.  In this scenario, it makes more sense to trade in your extra kits for FlexReads because FlexReads can be used with any kit, but SimpleSeq reactions no longer carry over between kits.

It is not a good option if you plan to distribute your kits out to different labs or individuals. For example, a purchasing manager might order 20 kits and hand them out to 20 people.  In this scenario, it is best to keep the kits.

Single Kit Owners - New vs. Old Process

Overflow -Graphic -for -old -vs -new ---1-Kit -v 2c

 

Issues with Old Process Benefits of New Process
  • When the first SimpleSeq kit is exhausted (0 reactions), there are leftover tubes.
  • The only way to use the leftover tubes was to call customer support to “reactivate” a tube.
  • Process requires more time and manual steps.
  • No need to call or "reactivate" tubes.  FlexReads are automatically added to the cart if needed.
  • Price is the same.
  • FlexReads can be used with any future kits.
  • Faster, easier and no more leftover tubes!

 

Overflow -Graphic -Legend

 

Using Existing Reactions

All of your existing reactions are still tied to your account.  Do not worry.   You may need to switch to a kit with available reactions before submitting your order though.   If you submit tube barcodes from an exhausted kit, you may see a “FlexRead” charge in the cart.  This is confusing to some users, because they may not even be aware that they are using an exhausted kit, or were previously taking reactions from other kits, due to the lack of transparency in the old system.   This confusion is easily resolved by looking at your sequencing dashboard.   The dashboard instantaneously shows which kits have reactions and which do not.  Avoid using kits with 0 reactions. 

Tips for Using the New System

  • If you see a charge for “FlexRead” in the cart, but have available reactions, it means you are submitting tubes from an exhausted kit. 
  • Switch to a kit with available reactions to easily resolve any issues in the cart.
  • Always visit your personal sequencing dashboard to check which kits have available reactions.

The new system improves the flexibility and traceablity of tubes, without impacting your existing reactions. The only difference is that you must submit orders from active kits and not exhausted kits (0 reactions).

 

  • No manual activation needed anymore!  Kits are automatically activated when shipped and viewable in your Sequencing Dashboard.
  • Sharing is now possible!

Sequencing is better shared. That is why SimpleSeq kits are now easier to share, activate and use. For instance, kits are now automatically activated when shipped so no manual activation step is required any longer. Also, anyone who submits a tube from a kit will be automatically ‘shared’ with that kit. Shared users can view the kit from their sequencing dashboard. The dashboard provides full transparency of who is using each and every tube and reaction in the kit.

  1. Kits are automatically activated when shipped now. There is no more need to manually activate a kit using the kit barcode.
  2. Kits are shareable. Barcoded tube orders can be submitted from anyone. Once the order is submitted, the new user can view the kit on their sequencing dashboard. For Premixed kits, the owner must manually share the kit using the share control in the Sequencing Dashboard.
  3. The Sequencing Dashboard is your new hub for tracking, viewing and managing everything related to SimpleSeq. You can view how many reactions and barcodes are available on your account. Also, it is possible to view the submission details for each individual tube from a visual map of the kit.
  4. Reactions will no longer overflow from one kit to another. Meaning reactions from one kit cannot be used with tubes from another kit.
  5. FlexReads are virtual reactions that can be used with any kit. They are a flexible and cost-friendly alternative to reactivation and overflowing between kits, both of which led to tracking issues in the past. More information can be found in the FlexRead section.
  6. Free barcodes no longer require manual activation. They are automatically activated once you enter them on the tube sequencing submission page.
  7. Reactivation has been replaced by FlexReads.
  8. It is possible to assign a kit to another user, which removes the kit from your account and applies a new owner to the kit. This is valuable for purchasing managers and lab managers. They can purchase a kit, then assign it to the end user.
  9. SimpleSeq Webless has been changed to SimpleSeq Premixed. The specifications of the product are unchanged.
  10. You can now assign and manually share a SimpleSeq Premixed kit with other users, using the Sequencing Dashboard controls.

Sharing Kits

Users can now quickly and easily share kits!  The person you wish to share with only needs to submit tube barcodes from your kit in an order and our system will automatically sync and share data between you both.  Alternatively, users can manually share a kit from the sequencing dashboard page.

 

 

 

Walkthrough for SimpleSeq

NOTE: SimpleSeq no longer requires manual activation. 

At the request of customers, we are happy to announce that SimpleSeq will now automatically activate to your account upon shipment.

 

 

Step SimpleSeq SimpleSeq Premixed

Laptopsml

Step 1

Order Kit

Both standard and premixed kits can be ordered on this page.

1.  You must be logged in.

2.  Order the SimpleSeq Kit (Standard or Premixed) using the control on the sidebar to the right.Simple Seq -ordering -control -v3

     

Kiticon

Step 2

Use Kit

Submit tube order online

1. Login to your account and go to the Sequencing order page,

2. Enter the barcode located on the side of your SimpleSeq tube into the "No. Barcode" field.  If the barcode is typed incorrectly or invalid, you may see an error message.

3. Fill in the other required fields.

4. In the cart, your SimpleSeq reactions go into our system at no cost.

Premixed Kits do not require online submission

SimpleSeq Premixed does not use the online order page.  It will not allow barcodes from premixed kits.  Please skip this step for premixed kits.

     

Dropboxsml

Step 3

Submit

Mail Your Samples Using the Free, Prepaid Shipping Labels

1. Print a copy of your online order and insert it into one of the free shipping envelopes that came with your kit.

2. Prepare your samples in tubes and load them into the same shipping envelope. For more information on preparing samples, go to the Sample Submissions Guideline.

3.  After defining the reaction, please drop them at a Eurofins Genomics dropbox (when available) or ship them via UPS overnight shipping evelopes provided with the SimpleSeq Kits. If you need additional envelopes, please order our free overnight shipping kits.

Mail Your Samples Using the Free, Prepaid Shipping Labels

To use the premixed kit, load your 'premixed' samples in the barcoded tubes through the split-seal caps and ship the samples to us. You do not need to submit the details of your samples submitted in the tubes from SimpleSeq Premixed kit online.

1) Prepare independent aqueous solutions of template and primer at the concentrations listed below.

2) Add template (8 μL) and primer (4 µL) to each SimpleSeqTM tube by inserting your pipet tip through the split seal (total volume = 12 µL).

3) Place tubes into a blue bag (supplied with the SimpleSeqTM kit) or custom ZipLock bags. Drop these samples at a Eurofins Genomics dropbox (when available) or ship them via UPS overnight shipping evelopes provided with the SimpleSeq Kits. If you need additional envelopes, please order our free overnight shipping kits.

     

 

Tips & More Information

  • You can drop off samples at any UPS dropbox for free, if using the free overnight envelopes supplied by us.
  • A copy of the online order confirmation page must be enclosed with every shipment to ensure proper identification, tracking, and result retrieval.
  • An alternative option would be to submit your samples into a nearby dropbox, if one is located near you.
  • Results are typically recieved by noon the following day, via a secure online link in your account.
  • For general sample preparation guidelines, please refer to the sample preparation guidelines.
  • After adding the kit to your cart, go to this page and order a free overnight shipping kit.  Although this overnight shipping kit is not required, we highly recommend it for new users.

Table 1: Components of SimpleSeqTM kits

Kits Include Standard Premixed
Free barcoded tubes with split seal caps for secure sample loading 96 pcs 96 pcs
Pre-paid, addressed UPS shipping envelopes 12 12
Barcoded Labels (use on tubes for sending primers) 32 pcs 0

The SimpleSeqTM Premixed kit is suitable for the submission of ‘premixed’ samples and removes the hassle of online submission. The SimpleSeqTM Standard kit allows for flexible and multiple sample submission options. You can pipette a template for multiple sequencing reactions into one SimpleSeqTM Standard barcoded tube. We’ll keep track of the number of sequencing ‘reactions’ you use. You can use the remaining barcoded SimpleSeqTM Standard tubes by simply purchasing flexpacks. The features of the two services are compared in table 2.

Table 2: Comparison of the SimpleSeqTM services

Features Standard Premixed
96-Sequencing reactions
Results delivered by noon, even on Sat. (no additional fee)  ✔
Website Interactions
Online results storage  ✔
User-friendly interface to manage/track inventory    ✔
Skip online submission  x  ✔
Online reaction submission and barcode reactivation    x
Sample submission and sequencing reaction options
Submit ‘premixed’ samples    ✔
Upgrade to power-read sequencing chemistry (additional fee)    x
Free standard primers    x
Custom primer synthesis (additional fee    x
Template for multiple sequencing reactions in one tube    x
Separate primer and template submission    x

aNote that the flexible sample submission options allows you to submit samples for more than 96 sequencing reactions using SimpleSeqTM standard kit; however, additional fees will apply for every read after 96 sequencing reactions.  

In summary, if you want to submit only ‘premixed’ samples, the SimpleSeqTM Premixed kit, which removes even the hassle of online submission, is the kit for you. If you want to avail the multiple benefits of flexible sample submission options, the SimpleSeqTM Standard kit and service, with flexible multiple options is what you need. We provide rapid overnight results for your sample, irrespective of the type of SimpleSeqTM kit used, shipped from anywhere in US, including Alaska and Hawaii, for the same low price!

Safe & Secure Sample Processing

  • Barcode scanned entries enabled
  • Verification of typed barcode entries
  • Barcode tracking of all samples throughout our process
  • LIMS workflow managed with Geospiza GeneSifterTM software
  • When submitted for a single reaction, no tube transfers ensure direct template-to-results correlation

Every step of the order entry process is validated to ensure that your order can be processed quickly and accurately—virtually eliminating the possibility of sample ID typos. Once your sample is received, Geospiza GeneSifter™ Lab Edition integrates your online order entry with each laboratory step and transfers results information for quality review and secure online retrieval by the same account that placed the order.

Eco-Friendly packaging

We use recyclable and/or recycled materials in our all of our packaging, marketing materials, and products. Look for the green recycling symbol on each product package for details.

  • Paperless sample submission—no need to print an order form
  • Recycled/recyclable shipping & packaging materials
  • Rack Recycling Program

When the rack is empty you can reuse it, send it to a local recycling center that accepts polypropylene, or return the racks to us for recycling.

Eurofins Genomics,
Rack Recycling Program 12701,
Plantside  Drive, Louisville, KY, 40299

Choose the tool that fits your needs!

Compare SimpleSeq Premixed and standard SimpleSeq below. Both guarantee results delivered the next morning, 96 reactions under 1 P.O., and you can submit tubes in any quantity at any time.

  Simple Seq -Premixed -Logo -for -web Simple Seq -Logo -for -web  
 
  • SIMPLE reaction submissions
  • Premix your sequencing then ship
  • No web submissions required
  • 1 premixed reaction per tube

Bottom line: Most convenient when ordering because no order forms are needed, just premix and ship.

  • Samples are not submited online. This saves you time but also means a slightly different process.
  • Submit template for multiple reactions in 1 tube
  • Full service or premixed reactions
  • Free standard primers

Bottom line: Most flexible and has better options for difficult templates.

 
       

Flex Read Logos _JSC_v 5 Done

 

FlexReads or 'Flexible' reads is implemented to give you more freedom when submitting samples using the SimpleSeqTM standard kit and replaces the process of 'reactivation'—wherein you had to call our customer service representative to manually 'reactivate' the barcode labels of your unused SimpleSeqTM tubes. FlexReads is a virtual product that can only be used only with SimpleSeqTM Standard kits. You can track its usage through your sequencing dashboard.

 

  • 1-11 reactions needed - the system will automatically add the exact number of reactions needed to the cart, instead of requiring a phone call to "reactivate" an old tube.
  • >11 reactions needed - the system will automatically add a FlexPack to the cart. FlexPack sizes include 12, 24, 48, 96

 

When can I use FlexReads:

When you have fewer SimpleSeq tubes than the number of sequencing reations you require. Consider that you have four unused SimpleSeq tubes with you and 4 unused reactions. Moreover, you have four plasmids that need to be sequenced and would like to run 8 sequencing reactions, two sequencing reactions for each template. You can then load up your samples into four tubes (amount sufficient for 2 sequencing rxns), order us to use the free standard primers and submit the order. You will automatically be charged for the 4 extra reads - these will appear as FlexReads on your order summary.    

You can also purchase these FlexReads prior to placing a sequencing order. The tally of your FlexReads in the sequencing dashboard will be incremented with the number of FlexReads or the quantity of the FlexPack you have purchased. Subsequently, when you assemble you samples in the tubes from the SimpleSeqTM Standard kit, and place the order, you not be charged until the SimpleSeq rxns and FlexReads are consumed.   

Please note that these FlexReads are not tied to a specific barcodes of the SimpleSeq standard kit; these FlexReads can be used any SimpleSeq Standard Kit.

Order FlexReads & Info